Biotechnology in Agriculture
Bishnu Bhusal
Introduction
Biotechnology is a newly emerging field of science having tremendous benefit to mankind. The government of most developed countries and some developing countries have emphasized in biotechnological ventures due to its potential impact for economic development in future. In a country like
In another word, 'biotechnology is the application of biological organism and molecules to technical and intestinal process (Prentiss, 1985)'.
Agriculture Biotechnology in
Biotechnology in the field of agriculture of
In the field of plant improvement, biotechnology in
(a) Government Sectors
Among the Government sectors laboratories, tissue culture laboratories of 'Department of Plant Resources (DPR)' and 'Nepal Agriculture Research council (NARC)' are prominent. DPR running pilot project of producing 1, 00,000 invitro plantlets of disease-free banana and citrus for distribution. Similarly for last several years, potato research programme under NARC has been producing virus free seeds of potato in its tissue culture lab and green house. Similarly two other division of NARC namely soil and plant pathology division have been engaged in research and development on biological nitrogen fixation using various species of Rhizobium and spawn production and distribution for mushroom culture using two genera Agrotis and Pleurotus. Recently NARC has also established tissue culture laboratories at some agricultural research station at Dhankuta and Lumle. A tissue culture laboratory is also established in horticulture development project in Kirtipur. Decision of establishment of Gene bank in NARC, in near future is one of the positive aspect in biotechnology field of
(b) Non Government Sector
Accompanied with government sector different non-government sector’s tissue culture laboratories has been established and some works are being carried out; some of the private sectors are as below
1) Botanical Enterprises Pvt. Ltd, Godawari
2)
3) Research Laboratory for Agriculture Biotechnology and Biodiversity (RLABB), Balkhu
4) Micro Plants, Kamal Pohkari.
5) Green Research and Technology (GREAT), Baneswor.
6)
Biotechnology and Plant Field
Applications of biotechnology for the improvement of crop in different aspects are discussed below in brief.
1) Clonal Propagation
Invitro multiplication of elites and high yielding types would be useful for clonal propagation of different crops and are used in producing new varieties in vegetatively propagated crops such as potato, sugarcane etc. The reason for invitro clonal propagations also include ease and efficiency of increase, maintenance of heterozygosity, sexual sterility and for incompatibility problems, Thus reforestation of
2) Pathogen Free Plants
By the application of biotechnology we are able to produce pathogen free plant. Exclusions and culture of the shoot meristem with only one or two leaf primordia often results in the physical elimination of virus (Karth 1984). Meristem-tip culture has also been used for elimination of bacteria, fungus from commercial plants such as carnations, gladioli; deffenbachia and nelargonium (Walkey 1978) are mentionable.
3) Germ Plasma Storage and Conservation
Biodiversity of many crops are lost each year by Neglect or mass eradication of their habitants for alternative uses. The objective of germ plasma conservation specifically of endangered, threatened and rare plants species is to ensure its availability in future for crop improvement. Thus by using biotechnology we can preserve genetic resources either in cryo preservation or gene bank which are used for crop improvement.
4) Somaclonal Variation
Somaclonal variation is one of the aspects of tissue culture technology and widely recommended for crop improvement especially of desired traits for drought, temperature and disease tolerance. Somaclonal variation is valuable source of new genetic information for breeders / geneticists which can be used in breeding purpose for crop improvement. Similarly protoclones or calliclones are produced from either single protoplast or single cells respectively and these often produced regenerated plants which show variation in phenotypes as compared to the original mother plant which is used for crop improvement.
5) Protoplast Culture or Fusion
Protoplast from different species can be induced to fuse by exposure to certain chemical or electric current. The resulting somatic hybrid may be grown invitro to produce callus tissue from which whole plant may be regenerated. Protoplast fusions help in crop improvement by somatic hybridization and cell modification to introduce novel gene or novel hybrid plant.
6) Production of Haploids by Anther Culture
The production of haploids and for spontaneous di-haploid plants through anther culture is extremely helpful in shortening the duration in varietals development. Haploids plants obtained either from anther culture or ovule culture may be grown normally under in-vitro condition up to flowering stage but viable gametes are not formed due to absence of onset of homologous chromosome and consequently there is no seed set. Application of haploids for crop improvement in plant breeding for releasing new varieties through fi-double-haploids system, selection of mutants resistant to disease, developing of asexual lines of trees/perennials species, transfer of desired alien genes etc.
Tissue Culture
Tissue culture technique has developed around the concept that a cell is totipotent that it has the capacity and ability to develop into whole organism. Explants can be obtained from inter-organ, inter-tissue and inter-cellular interactions and subjected to direct experimental control. Due to playing with plant tissues in laboratory this technique has been referred by some researchers as a 'botanical laser' whose numerous uses are yet to be fully understood.
Application of tissue culture techniques for improvement of crop of is shown in diagram below.
Clonal Propagation
Application in Animal Field
1) Artificial Insemination
A male animal produces millions of sperms daily. Theoretically it can be inseminate females regularly and produce several offspring's. This excess capacity of male has been utilized through developing new technologies for artificial insemination which can be said as the first biotechnology. In context of Nepal AI techniques is gaining popularity day-by-day.
2) Transgenic Animals
It involves the gene from one animal, modification of them in lab and introduction of them into animals of same or different species by using biotechnology and genetic engineering techniques. Transgenic animals have been instrumental in providing new insight into mechanism of development and developmental gene regulation, into the action of oncogenes and into the intricate cell interactions within the immune system.
Areas of possible investigation of domestic livestock by using the transgenic technology have been given in figure.
Milk Blood
* Increase production * Pharmaceuticals
* Milk addition * Circulating peptides
* Pharmaceuticals * D/S resistance
* Extraction
TRANSGENIC LIVESTOCK.
* By product * Hormones
* Feather * Releasing factors
*Wool * Neuropathies
(Source: A Text Book of Biotechnology R.C. Dubey, 2006, page no. 266)
3) Embryo transfer (ET)
ET is technique by which embryos (fertilized ova) are collected from the reproductive tract of a female animal prior nidation and transplanted into the reproductive tract of other animal to complete their gestation i.e. conception, implantation and delivery.
Applications of ET
a) New breeding strategies.
b) Reduction of the period of the animal improvement.
c) Higher replication rate of valuable genotype.
d) Fostering mother transfer immunity.
e) Conservation and utilization of genetic resources.
f) Study of reproductive disease.
4) Invitro-Fertilization (IVF) technology
The term 'invitro' means in glass or in artificial conditions and IVF refers to the fact that fertilization of egg by sperm had occurred not in uterus but outside the uterus at artificially maintained optimum condition. In recent years the IVF technology has revolutionized the field of animal biotechnology because of production of more and more animals as compared to animal production through normal cases.
5) Fermentation
Fermentation is defined as 'any anaerobic process for the production of useful products through mass culture of micro-organisms' where as in a biochemical sense this word means the numerous oxidation reduction reactions in which organic compounds used as a source of carbon and energy. Besides productions of beverages, food, fermentation technology is also used to produce major veterinary by products from microbial fermentation such as antibiotics, disease diagnostic reagents.
6) Vaccine production
Vaccine is chemical substances prepared from the proteins (antigen) of other animals which confer immunity to a particular vinrus. Vaccines may be live vaccines, killed vaccines, inactivated vaccines, sub-unit vaccines, DNA vaccines etc. For the synthesis of these types of vaccines biotechnological principles is used such as vaccines for Rabies virus (RV), vaccines for FMD virus (FMOV)
Other different possible application of biotechnology is animal field are.
7) Pregnancy Diagnosis.
8) Antibodies Tissue Culture and Cell Cultures.
9) Application of Molecular Markers in Improvement of Livestock.
10) Hormone Production.
11) Clinical Practices such as ELISA, MRI etc.
Conclusion
Applications of biotechnology in agriculture have been given more emphasize and up to some extent application of biotechnology will be a valuable tool in solving problems of hunger, energy supply, improving quality of life and to reduce environmental pollution. However in
References
Dubey, RC 2006, A Text Book of Biotechnology, S.Chand & Company Ltd.
Molecular Biology, Biotechnology and Environment, 1997
Ranjit, M 2007, 'Agriculture Biotechnology in
Bishnu Bhusal 'Dangali'
B.Sc. (Hons) Ag. 5th semester